Ignore the chaos in the background. 😅 My dog came through and knocked everything over with the wiggles.
Extremely excited about the progress of my Paraiso verde. It was a tissue culture start.
Ignore the chaos in the background. 😅 My dog came through and knocked everything over with the wiggles.
12 Comments
HeavyMitchel@lemmy.ml · 7 pts · 3y
Nice ! Are you certain the cat has nothing to do with the chaos ? They're well versed in the art of framing the dog.
fossilesque@mander.xyz · 4 pts · 3y
vicfic@iusearchlinux.fyi · 2 pts · 3y
Which camera did you use? Like, ik its not relevant but the pic is too good.
fossilesque@mander.xyz · 3 pts · 3y
photonic_sorcerer@lemmy.dbzer0.com · 2 pts · 3y
Hi! Love the plant. What's a tissue culture start?
fossilesque@mander.xyz · 2 pts · 3y
karmiclychee@sh.itjust.works · 1 pts · 3y
That's intense
Sal@mander.xyz · 1 pts · 3y
😱 Cool! This smells like the start of a new hobby....
Doxatek@mander.xyz · 2 pts · 3y
Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you
Sal@mander.xyz · 1 pts · 3y
Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I'm thinking some of these skills might transfer when I do get into it. A reason why I'm interested in this is because some of the work I've read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don't really know how one would practically achieve that. So I'm very curious about it...
Doxatek@mander.xyz · 1 pts · 3y
Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don't have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.
Really cool you've already done it at home though. Did you do pressure cooker method
Sal@mander.xyz · 2 pts · 3y
Oh, I haven't worked with hormones before. That would be interesting.
Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.
Doxatek@mander.xyz · 2 pts · 3y
Oh yeah the hormones are cool you can make them do some weird stuff haha
Botrychium@mander.xyz · 1 pts · 3y
nice one! did you do the tissue culture yourself?
fossilesque@mander.xyz · 2 pts · 3y